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Quantitative method of measuring phosphatidylserine externalization during apoptosis using electron paramagnetic resonance (EPR) spectroscopy and annexin-conjugated iron.
Fabisiak, James P; Borisenko, Grigory G; Kagan, Valerian E.
Afiliación
  • Fabisiak JP; Department of Environmental and Occupational Health, University of Pittsburgh, 100 Technology Drive, Bridgeside Point, Pittsburgh, PA, 15219, USA, fabs@pitt.edu.
Methods Mol Biol ; 1105: 613-21, 2014.
Article en En | MEDLINE | ID: mdl-24623256
We present here the application of a novel assay that measures the absolute amount of PS externalized on the surface of cells. While based on the same annexin binding principle as the fluorescent flow cytometry assay, we use paramagnetic iron as the ultimate reporter molecule, establishing a linear relationship between signal amplitude and amount of PS on the cell surface, allowing a quantitative assay of PS externalization over a wide dynamic range. The application of this technique, alone and in concert with the PS oxidation method presented in the previous chapter, will greatly aid in studying the mechanistic connection between lipid peroxidation and translocation events during apoptosis.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatidilserinas / Apoptosis / Anexina A5 / Hierro Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatidilserinas / Apoptosis / Anexina A5 / Hierro Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos