Crystallization and preliminary X-ray crystallographic analysis of the catalytic domain of membrane type 1 matrix metalloproteinase.
Acta Crystallogr F Struct Biol Commun
; 70(Pt 2): 232-5, 2014 Feb.
Article
en En
| MEDLINE
| ID: mdl-24637763
ABSTRACT
Membrane type 1 matrix metalloproteinase (MT1-MMP) belongs to the large family of zinc-dependent endopeptidases termed MMPs that are located in the extracellular matrix. MT1-MMP was crystallized at 277 K using the vapour-diffusion method with PEG as a precipitating agent. Data sets for MT1-MMP were collected to 2.24 Å resolution at 100 K. The crystals belonged to space group P4(3)2(1)2, with unit-cell parameters a = 62.99, c = 122.60 Å. The crystal contained one molecule per asymmetric unit, with a Matthews coefficient (VM) of 2.90 Å(3) Da(-1); the solvent content is estimated to be 57.6%.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cristalografía por Rayos X
/
Metaloproteinasa 1 de la Matriz
Límite:
Humans
Idioma:
En
Revista:
Acta Crystallogr F Struct Biol Commun
Año:
2014
Tipo del documento:
Article
País de afiliación:
Alemania