Expression, purification, and crystallization of HSV-1 glycoproteins for structure determination.
Methods Mol Biol
; 1144: 249-63, 2014.
Article
en En
| MEDLINE
| ID: mdl-24671689
ABSTRACT
HSV glycoproteins play important roles in the viral infectious cycle, particularly viral entry into the cell. Here we describe the protocol for expression, purification, and crystallization of viral glycoproteins based on those developed for the HSV-1 gB and HSV-2 gH/gL ectodomains. These protocols can be used for generating milligram amounts of wild-type (WT) or mutant gB and gH/gL ectodomains or can be adapted to produce purified ectodomains of other HSV glycoproteins for biochemical and structural studies.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Glicoproteínas
/
Herpesvirus Humano 2
/
Herpesvirus Humano 1
/
Biología Molecular
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos