Dual-polarization Raman spectral imaging to extract overlapping molecular fingerprints of living cells.
J Biophotonics
; 8(7): 546-54, 2015 Jul.
Article
en En
| MEDLINE
| ID: mdl-24733812
ABSTRACT
Raman spectral imaging is gaining more and more attention in biological studies because of its label-free characteristic. However, the discrimination of overlapping chemical contrasts has been a major challenge. In this study, we introduce an optical method to simultaneously obtain two orthogonally polarized Raman images from a single scan of the sample. We demonstrate how this technique can improve the quality and quantity of the hyperspectral Raman dataset and how the technique is expected to further extend the horizons of Raman spectral imaging in biological studies by providing more detailed chemical information. The dual-polarization Raman images of a HeLa cell.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría Raman
/
Procesamiento de Imagen Asistido por Computador
/
Imagen Molecular
/
Microscopía
Límite:
Humans
Idioma:
En
Revista:
J Biophotonics
Asunto de la revista:
BIOFISICA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Japón