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Measuring microtubule polarity in spindles with second-harmonic generation.
Yu, Che-Hang; Langowitz, Noah; Wu, Hai-Yin; Farhadifar, Reza; Brugues, Jan; Yoo, Tae Yeon; Needleman, Daniel.
Afiliación
  • Yu CH; School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts. Electronic address: chehangyu@fas.harvard.edu.
  • Langowitz N; School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts.
  • Wu HY; Department of Physics, Harvard University, Cambridge, Massachusetts.
  • Farhadifar R; School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts; Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts; FAS Center for Systems Biology, Harvard University, Cambridge, Massachusetts.
  • Brugues J; School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts; Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts; FAS Center for Systems Biology, Harvard University, Cambridge, Massachusetts; Max Planck Institute of Molecular Cell B
  • Yoo TY; School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts.
  • Needleman D; School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts; Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts; FAS Center for Systems Biology, Harvard University, Cambridge, Massachusetts.
Biophys J ; 106(8): 1578-87, 2014 Apr 15.
Article en En | MEDLINE | ID: mdl-24739157
The spatial organization of microtubule polarity, and the interplay between microtubule polarity and protein localization, is thought to be crucial for spindle assembly, anaphase, and cytokinesis, but these phenomena remain poorly understood, in part due to the difficulty of measuring microtubule polarity in spindles. We develop and implement a method to nonperturbatively and quantitatively measure microtubule polarity throughout spindles using a combination of second-harmonic generation and two-photon fluorescence. We validate this method using computer simulations and by comparison to structural data on spindles obtained from electron tomography and laser ablation. This method should provide a powerful tool for studying spindle organization and function, and may be applicable for investigating microtubule polarity in other systems.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Simulación por Computador / Polaridad Celular / Microtúbulos / Huso Acromático Límite: Animals Idioma: En Revista: Biophys J Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Simulación por Computador / Polaridad Celular / Microtúbulos / Huso Acromático Límite: Animals Idioma: En Revista: Biophys J Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos