Genome-wide profiling of yeast DNA:RNA hybrid prone sites with DRIP-chip.
PLoS Genet
; 10(4): e1004288, 2014 Apr.
Article
en En
| MEDLINE
| ID: mdl-24743342
ABSTRACT
DNARNA hybrid formation is emerging as a significant cause of genome instability in biological systems ranging from bacteria to mammals. Here we describe the genome-wide distribution of DNARNA hybrid prone loci in Saccharomyces cerevisiae by DNARNA immunoprecipitation (DRIP) followed by hybridization on tiling microarray. These profiles show that DNARNA hybrids preferentially accumulated at rDNA, Ty1 and Ty2 transposons, telomeric repeat regions and a subset of open reading frames (ORFs). The latter are generally highly transcribed and have high GC content. Interestingly, significant DNARNA hybrid enrichment was also detected at genes associated with antisense transcripts. The expression of antisense-associated genes was also significantly altered upon overexpression of RNase H, which degrades the RNA in hybrids. Finally, we uncover mutant-specific differences in the DRIP profiles of a Sen1 helicase mutant, RNase H deletion mutant and Hpr1 THO complex mutant compared to wild type, suggesting different roles for these proteins in DNARNA hybrid biology. Our profiles of DNARNA hybrid prone loci provide a resource for understanding the properties of hybrid-forming regions in vivo, extend our knowledge of hybrid-mitigating enzymes, and contribute to models of antisense-mediated gene regulation. A summary of this paper was presented at the 26th International Conference on Yeast Genetics and Molecular Biology, August 2013.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN de Hongos
/
ARN de Hongos
/
Regulación Fúngica de la Expresión Génica
/
Hibridación de Ácido Nucleico
Idioma:
En
Revista:
PLoS Genet
Asunto de la revista:
GENETICA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Canadá