Effects of ammonium on the activity and community of methanotrophs in landfill biocover soils.
Syst Appl Microbiol
; 37(4): 296-304, 2014 Jun.
Article
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| MEDLINE
| ID: mdl-24794017
ABSTRACT
The influence of NH4(+) on microbial CH4 oxidation is still poorly understood in landfill cover soils. In this study, effects of NH4(+) addition on the activity and community structure of methanotrophs were investigated in waste biocover soil (WBS) treated by a series of NH4(+)-N contents (0, 100, 300, 600 and 1200mgkg(-1)). The results showed that the addition of NH4(+)-N ranging from 100 to 300mgkg(-1) could stimulate CH4 oxidation in the WBS samples at the first stage of activity, while the addition of an NH4(+)-N content of 600mgkg(-1) had an inhibitory effect on CH4 oxidation in the first 4 days. The decrease of CH4 oxidation rate observed in the last stage of activity could be caused by nitrogen limitation and/or exopolymeric substance accumulation. Type I methanotrophs Methylocaldum and Methylobacter, and type II methanotrophs (Methylocystis and Methylosinus) were abundant in the WBS samples. Of these, Methylocaldum was the main methanotroph in the original WBS. With incubation, a higher abundance of Methylobacter was observed in the treatments with NH4(+)-N contents greater than 300mgkg(-1), which suggested that NH4(+)-N addition might lead to the dominance of Methylobacter in the WBS samples. Compared to type I methanotrophs, the abundance of type II methanotrophs Methylocystis and/or Methylosinus was lower in the original WBS sample. An increase in the abundance of Methylocystis and/or Methylosinus occurred in the last stage of activity, and was likely due to a nitrogen limitation condition. Redundancy analysis showed that NH4(+)-N and the C/N ratio had a significant influence on the methanotrophic community in the WBS sample.
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Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microbiología del Suelo
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Methylococcaceae
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Methylocystaceae
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Biota
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Compuestos de Amonio
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Metano
Idioma:
En
Revista:
Syst Appl Microbiol
Año:
2014
Tipo del documento:
Article
País de afiliación:
China