Measurement of the temperature of the resting rotor in analytical ultracentrifugation.
Anal Biochem
; 458: 37-9, 2014 Aug 01.
Article
en En
| MEDLINE
| ID: mdl-24799348
ABSTRACT
Accurate measurements of rotor temperature are critical for the interpretation of hydrodynamic parameters in analytical ultracentrifugation. We have recently developed methods for a more accurate determination of the temperature of a spinning rotor using iButton temperature loggers. Here we report that the temperature measured with the iButton on the counterbalance of a resting rotor, following thermal equilibration under high vacuum, closely corresponded to the temperature of the spinning rotor with a precision better than 0.2°C. This strategy offers an inexpensive and straightforward approach to monitor the accuracy of the temperature calibration and determine corrective temperature offsets.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ultracentrifugación
Idioma:
En
Revista:
Anal Biochem
Año:
2014
Tipo del documento:
Article