Pseudotyping of vesicular stomatitis virus with the envelope glycoproteins of highly pathogenic avian influenza viruses.
J Gen Virol
; 95(Pt 8): 1634-1639, 2014 Aug.
Article
en En
| MEDLINE
| ID: mdl-24814925
ABSTRACT
Pseudotype viruses are useful for studying the envelope proteins of harmful viruses. This work describes the pseudotyping of vesicular stomatitis virus (VSV) with the envelope glycoproteins of highly pathogenic avian influenza viruses. VSV lacking the homotypic glycoprotein (G) gene (VSVΔG) was used to express haemagglutinin (HA), neuraminidase (NA) or the combination of both. Propagation-competent pseudotype viruses were only obtained when HA and NA were expressed from the same vector genome. Pseudotype viruses containing HA from different H5 clades were neutralized specifically by immune sera directed against the corresponding clade. Fast and sensitive reading of test results was achieved by vector-mediated expression of GFP. Pseudotype viruses expressing a mutant VSV matrix protein showed restricted spread in IFN-competent cells. This pseudotype system will facilitate the detection of neutralizing antibodies against virulent influenza viruses, circumventing the need for high-level biosafety containment.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus de la Influenza A
/
Proteínas Virales
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Glicoproteínas Hemaglutininas del Virus de la Influenza
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Anticuerpos Neutralizantes
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Técnicas de Visualización de Superficie Celular
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Gripe Aviar
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Anticuerpos Antivirales
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Neuraminidasa
Límite:
Animals
Idioma:
En
Revista:
J Gen Virol
Año:
2014
Tipo del documento:
Article
País de afiliación:
Suiza