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Neonatal pial surface electroporation.
Levy, Rachelle; Molina, Jessica; Danielpour, Moise; Breunig, Joshua J.
Afiliación
  • Levy R; Department of Neurosurgery, Regenerative Medicine Institute, Cedars-Sinai Medical Center.
  • Molina J; Department of Biomedical Sciences, Regenerative Medicine Institute, Cedars-Sinai Medical Center.
  • Danielpour M; Department of Neurosurgery, Regenerative Medicine Institute, Cedars-Sinai Medical Center.
  • Breunig JJ; Department of Biomedical Sciences, Regenerative Medicine Institute, Cedars-Sinai Medical Center; joshua.breunig@cshs.org.
J Vis Exp ; (87)2014 May 07.
Article en En | MEDLINE | ID: mdl-24836046
Over the past several years the pial surface has been identified as a germinal niche of importance during embryonic, perinatal and adult neuro- and gliogenesis, including after injury. However, methods for genetically interrogating these progenitor populations and tracking their lineages had been limited owing to a lack of specificity or time consuming production of viruses. Thus, progress in this region has been relatively slow with only a handful of investigations of this location. Electroporation has been used for over a decade to study neural stem cell properties in the embryo, and more recently in the postnatal brain. Here we describe an efficient, rapid, and simple technique for the genetic manipulation of pial surface progenitors based on an adapted electroporation approach. Pial surface electroporation allows for facile genetic labeling and manipulation of these progenitors, thus representing a time-saving and economical approach for studying these cells.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piamadre / Electroporación Límite: Animals Idioma: En Revista: J Vis Exp Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piamadre / Electroporación Límite: Animals Idioma: En Revista: J Vis Exp Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos