Aqueous extract of unfermented honeybush (Cyclopia maculata) attenuates STZ-induced diabetes and ß-cell cytotoxicity.
Planta Med
; 80(8-9): 622-9, 2014 Jun.
Article
en En
| MEDLINE
| ID: mdl-24853761
ABSTRACT
New strategies, which include ß-cell protection, are required in the treatment of T2D, as current drugs demonstrate little or no capacity to directly protect the vulnerable ß-cell against diabetes-induced cytotoxicity. In this study we investigated the ameliorative effect of pre-treatment with an aqueous extract of unfermented Cyclopia maculata (honeybush) on STZ-induced diabetes and pancreatic ß-cell cytotoxicity in Wistar rats after demonstrating a protective effect in vitro in RIN-5F cells. The amelioration of STZ-induced diabetes was seen in the reduction of the area under the curve, determined by the oral glucose tolerance test, as well as fasting glucose levels in extract-treated rats. Pre-treatment with extract also improved serum triglyceride levels and the glucose-to-insulin ratio. Pre-treatment with the extract or the drug, metformin, increased the ß-cell area in islets, with a concomitant increase in ß-cell proliferation at the higher extract dose (300 mg/kg/d), but not the lower dose (30 mg/kg/d). Subsequently, the in vitro tritiated thymidine incorporation assay showed that the extract was not mitogenic in RIN-5F cells. STZ-induced elevation of plasma nitrite levels was reduced in extract-treated rats, but no changes were observed in their serum catalase, serum glutathione, liver lipid peroxidation and liver nitrotyrosine levels. Pre-treating the rats with extract ameliorated the diabetic effect of STZ in Wistar rats, with evidence of pancreatic ß-cells protection, attributed to the presence of high levels of antioxidants such as the xanthones, mangiferin and isomangiferin.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Xantonas
/
Diabetes Mellitus Experimental
/
Células Secretoras de Insulina
/
Cyclopia (Planta)
/
Antioxidantes
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Planta Med
Año:
2014
Tipo del documento:
Article
País de afiliación:
Sudáfrica