Ultrasensitive enzyme-free immunoassay for squamous cell carcinoma antigen using carbon supported Pd-Au as electrocatalytic labels.
Anal Chim Acta
; 833: 9-14, 2014 Jun 23.
Article
en En
| MEDLINE
| ID: mdl-24909768
ABSTRACT
A novel nonenzymatic sandwich-type electrochemical immunosensor has been developed to detect squamous cell carcinoma antigen (SCCA). Nitrogen-doped graphene sheet (N-GS) was used to increase capacity of capturing primary antibodies (Ab1). Carbon-supported Pd-Au binary nanoparticles (Pd-Au/C) were synthesized and used to label secondary antibodies (Ab2). The specific binding of SCCA and antibodies enabled a quantitative attachment of Pd-Au/C on the electrode surface. Electrocatalytic analysis showed that the prepared Pd-Au/C exhibit excellent electrocatalytic activity towards hydrogen peroxide (H2O2). We use current response of electrocatalytic labels Pd-Au/C to detect the concentration of SCCA. The unique nonenzymatic immunosensor exhibits a relatively wide linear range from 0.005 to 2 ng mL(-1) and high sensitivity with a low detection limit of 1.7 pg mL(-1). The immunsensor also shows good reproducibility (4.2%) and stability (5.8%), which makes it an enormous application prospect in clinical research.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Paladio
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Inmunoensayo
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Carbono
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Serpinas
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Oro
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Antígenos de Neoplasias
Idioma:
En
Revista:
Anal Chim Acta
Año:
2014
Tipo del documento:
Article
País de afiliación:
China