Extraction and purification of polysaccharides from pine medicinal mushroom, Tricholoma matsutake (higher Basidiomycetes) fruit bodies.
Int J Med Mushrooms
; 16(2): 149-60, 2014.
Article
en En
| MEDLINE
| ID: mdl-24941036
ABSTRACT
Ultrasound-assisted extraction, purification, and characterization of antioxidant polysaccharides from Tricholoma matsutake were carried out. On the basis of the results of a single-factor test, three independent and main variables, including extraction time (X1 130-170 s), ultrasonic power (X2 340-380 W), and ratio of water to raw material (X3 45-55 mL/g) were studied by Box-Behnken design. The optimum extraction conditions are as follows extraction time 160 s, ultrasonic power 365 W, and ratio of water to raw material 53.5 mL/g. Under the optimized conditions, the yield of T. matsutake polysaccharides (TMP) was 7.97±0.31%. The crude TMP was purified by DEAE-cellulose 52 chromatography and Sephadex G-100 chromatography to afford two fractions, TMP-1 and TMP-2. The crude TMP contained 85.76% carbohydrates, 3.57% proteins, and 0.13% uronic acids. The constituent monosaccharides were predominantly glucose, galactose, mannose, xylose, fucose, and glucuronic acid residues.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacáridos
/
Verduras
/
Extractos Vegetales
/
Cuerpos Fructíferos de los Hongos
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Tricholoma
Idioma:
En
Revista:
Int J Med Mushrooms
Año:
2014
Tipo del documento:
Article