Towards tender X-rays with Zernike phase-contrast imaging of biological samples at 50 nm resolution.
J Synchrotron Radiat
; 21(Pt 4): 790-4, 2014 Jul.
Article
en En
| MEDLINE
| ID: mdl-24971976
ABSTRACT
X-ray microscopy is a commonly used method especially in material science application, where the large penetration depth of X-rays is necessary for three-dimensional structural studies of thick specimens with high-Z elements. In this paper it is shown that full-field X-ray microscopy at 6.2â
keV can be utilized for imaging of biological specimens with high resolution. A full-field Zernike phase-contrast microscope based on diffractive optics is used to study lipid droplet formation in hepatoma cells. It is shown that the contrast of the images is comparable with that of electron microscopy, and even better contrast at tender X-ray energies between 2.5â
keV and 4â
keV is expected.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Difracción de Rayos X
/
Interpretación de Imagen Radiográfica Asistida por Computador
/
Intensificación de Imagen Radiográfica
/
Tomografía Computarizada por Rayos X
/
Microscopía de Contraste de Fase
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Synchrotron Radiat
Asunto de la revista:
RADIOLOGIA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Suiza