Improved performance of the PacBio SMRT technology for 16S rDNA sequencing.
J Microbiol Methods
; 104: 59-60, 2014 Sep.
Article
en En
| MEDLINE
| ID: mdl-24978594
ABSTRACT
Improved sequencing accuracy was obtained with 16S amplicons from environmental samples and a known pure culture when upgraded Pacific Biosciences (PacBio) hardware and enzymes were used for the single molecule, real-time (SMRT) sequencing platform. The new PacBio RS II system with P4/C2 chemistry, when used with previously constructed libraries (Mosher et al., 2013) surpassed the accuracy of Roche/454 pyrosequencing platform. With accurate read lengths of >1400 base pairs, the PacBio system opens up the possibility of identifying microorganisms to the species level in environmental samples.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN Bacteriano
/
ADN Ribosómico
/
ARN Ribosómico 16S
/
Shewanella
/
Secuenciación de Nucleótidos de Alto Rendimiento
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
J Microbiol Methods
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos