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Characterization and functional properties of gastric tissue-resident memory T cells from children, adults, and the elderly.
Booth, Jayaum S; Toapanta, Franklin R; Salerno-Goncalves, Rosangela; Patil, Seema; Kader, Howard A; Safta, Anca M; Czinn, Steven J; Greenwald, Bruce D; Sztein, Marcelo B.
Afiliación
  • Booth JS; Center for Vaccine Development, University of Maryland School of Medicine , Baltimore, MD , USA ; Department of Pediatrics, University of Maryland School of Medicine , Baltimore, MD , USA.
  • Toapanta FR; Center for Vaccine Development, University of Maryland School of Medicine , Baltimore, MD , USA ; Department of Medicine, University of Maryland School of Medicine , Baltimore, MD , USA.
  • Salerno-Goncalves R; Center for Vaccine Development, University of Maryland School of Medicine , Baltimore, MD , USA ; Department of Pediatrics, University of Maryland School of Medicine , Baltimore, MD , USA.
  • Patil S; Department of Medicine, University of Maryland School of Medicine , Baltimore, MD , USA ; Division of Gastroenterology and Hepatology, University of Maryland School of Medicine , Baltimore, MD , USA.
  • Kader HA; Department of Pediatrics, University of Maryland School of Medicine , Baltimore, MD , USA.
  • Safta AM; Department of Pediatrics, University of Maryland School of Medicine , Baltimore, MD , USA.
  • Czinn SJ; Department of Pediatrics, University of Maryland School of Medicine , Baltimore, MD , USA.
  • Greenwald BD; Department of Medicine, University of Maryland School of Medicine , Baltimore, MD , USA ; Division of Gastroenterology and Hepatology, University of Maryland School of Medicine , Baltimore, MD , USA.
  • Sztein MB; Center for Vaccine Development, University of Maryland School of Medicine , Baltimore, MD , USA ; Department of Pediatrics, University of Maryland School of Medicine , Baltimore, MD , USA ; Department of Medicine, University of Maryland School of Medicine , Baltimore, MD , USA.
Front Immunol ; 5: 294, 2014.
Article en En | MEDLINE | ID: mdl-24995010
ABSTRACT
T cells are the main orchestrators of protective immunity in the stomach; however, limited information on the presence and function of the gastric T subsets is available mainly due to the difficulty in recovering high numbers of viable cells from human gastric biopsies. To overcome this shortcoming we optimized a cell isolation method that yielded high numbers of viable lamina propria mononuclear cells (LPMC) from gastric biopsies. Classic memory T subsets were identified in gastric LPMC and compared to peripheral blood mononuclear cells (PBMC) obtained from children, adults, and the elderly using an optimized 14 color flow cytometry panel. A dominant effector memory T (TEM) phenotype was observed in gastric LPMC CD4(+) and CD8(+) T cells in all age groups. We then evaluated whether these cells represented a population of gastric tissue-resident memory T (TRM) cells by assessing expression of CD103 and CD69. The vast majority of gastric LPMC CD8(+) T cells either co-expressed CD103/CD69 (>70%) or expressed CD103 alone (~20%). Gastric LPMC CD4(+) T cells also either co-expressed CD103/CD69 (>35%) or expressed at least one of these markers. Thus, gastric LPMC CD8(+) and CD4(+) T cells had the characteristics of TRM cells. Gastric CD8(+) and CD4(+) TRM cells produced multiple cytokines (IFN-γ, IL-2, TNF-α, IL-17A, MIP-1ß) and up-regulated CD107a upon stimulation. However, marked differences were observed in their cytokine and multi-cytokine profiles when compared to their PBMC TEM counterparts. Furthermore, gastric CD8(+) TRM and CD4(+) TRM cells demonstrated differences in the frequency, susceptibility to activation, and cytokine/multi-cytokine production profiles among the age groups. Most notably, children's gastric TRM cells responded differently to stimuli than gastric TRM cells from adults or the elderly. In conclusion, we demonstrate the presence of gastric TRM, which exhibit diverse functional characteristics in children, adults, and the elderly.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Front Immunol Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Front Immunol Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos