Damage and recovery of the ovary in female zebrafish i.p.-injected with MC-LR.

Up to now, in vivo studies on toxic effects of microcystins (MCs) on the reproductive system are limited and the underlying molecular mechanisms of MCs-induced reproductive toxicity remain to be elucidated. In an acute toxic experiment, female zebrafish (Danio rerio) were injected intraperitoneally (i.p.) at doses of 50 and 200 µg MC-LR/kg body weight (BW) respectively, and histopathological lesions and antioxidant enzymatic activities and gene expression in the ovary were studied at 1, 3, 12, 24, 48 and 168 h post injection (hpi). Pathological lesions of zebrafish ovary progressed in severity and extent with the increasing exposure time and dose within 12 hpi. Concurrently, the increases in malondialdehyde (MDA) contents as well as the enzymatic activities and transcriptional levels of antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) showed the occurrence of oxidative stress, indicating that MC-LR induced adverse effects on the structure and functional activity of zebrafish ovary. Oxidative stress plays a significant role in the reproductive toxicity of MC-LR. The significant decrease of glutathione (GSH) content in zebrafish ovary suggested the importance of MC-LR detoxification by glutathione S-transferases (GST) via GSH. The final recovery of histostructure and antioxidative indices indicated that ovarian efficient antioxidant defense system might be an important mechanism of zebrafish to counteract MC-LR. Although the negative effects of MC-LR can be overcome by ovarian antioxidant system in this study, the potential reproductive risks of MC-LR should not be neglected because of its wide occurrence.