Metabolomics investigation of cutaneous T cell lymphoma based on UHPLC-QTOF/MS.
Asian Pac J Cancer Prev
; 15(13): 5417-21, 2014.
Article
en En
| MEDLINE
| ID: mdl-25041011
ABSTRACT
OBJECTIVES:
The identification of cutaneous T cell lymphoma (CTCL) biomarkers may serve as a predictor of disease progression and treatment response. The aim of this study was to map potential biomarkers in CTCL plasma. DESIGN ANDMETHODS:
Plasma metabolic perturbations between CTCL cases and healthy individuals were investigated using metabolomics and ultrahigh performance liquid chromatographyquadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS).RESULTS:
Principal component analysis (PCA) of the spectra showed clear metabolic changes between the two groups. Thirty six potential biomarkers associated with CTCL were found.CONCLUSIONS:
Based on PCA, several biomarkers were determined and further identified by LC/MS/MS analysis. All of these could be potential early markers of CTCL. In addition, we established that heparin as anticoagulant has better pre-treatment results than EDTA with the UHPLC-QTOF/MS approach.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Linfoma Cutáneo de Células T
/
Metaboloma
Tipo de estudio:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Asian Pac J Cancer Prev
Asunto de la revista:
NEOPLASIAS
Año:
2014
Tipo del documento:
Article