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Induction and suppression of tick cell antiviral RNAi responses by tick-borne flaviviruses.
Schnettler, Esther; Tykalová, Hana; Watson, Mick; Sharma, Mayuri; Sterken, Mark G; Obbard, Darren J; Lewis, Samuel H; McFarlane, Melanie; Bell-Sakyi, Lesley; Barry, Gerald; Weisheit, Sabine; Best, Sonja M; Kuhn, Richard J; Pijlman, Gorben P; Chase-Topping, Margo E; Gould, Ernest A; Grubhoffer, Libor; Fazakerley, John K; Kohl, Alain.
Afiliación
  • Schnettler E; MRC - University of Glasgow Centre for Virus Research, Glasgow G11 5JR, UK The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK Esther.Schnettler@glasgow.ac.uk.
  • Tykalová H; Faculty of Science, University of South Bohemia and Biology Centre, Institute of Parasitology, Czech Academy of Sciences, 37005 Ceské Budejovice (Budweis), Czech Republic.
  • Watson M; The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK.
  • Sharma M; Markey Centre for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette IN 47907, USA.
  • Sterken MG; Laboratory of Virology, Wageningen University, 6708 PB Wageningen, The Netherlands.
  • Obbard DJ; Institute of Evolutionary Biology and Centre for Infection Immunity and Evolution, University of Edinburgh, EH9 3JT, UK.
  • Lewis SH; Institute of Evolutionary Biology and Centre for Infection Immunity and Evolution, University of Edinburgh, EH9 3JT, UK.
  • McFarlane M; MRC - University of Glasgow Centre for Virus Research, Glasgow G11 5JR, UK.
  • Bell-Sakyi L; The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK.
  • Barry G; The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK.
  • Weisheit S; The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK.
  • Best SM; Innate Immunity and Pathogenesis Unit, Laboratory of Virology, Rocky Mountain Laboratories, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA.
  • Kuhn RJ; Markey Centre for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette IN 47907, USA.
  • Pijlman GP; Laboratory of Virology, Wageningen University, 6708 PB Wageningen, The Netherlands.
  • Chase-Topping ME; Centre for Immunity, Infection and Evolution, University of Edinburgh, EH9 3JT, UK.
  • Gould EA; Unité des Virus Emergents, Faculté de Médicine Timone, 13385 Marseille Cedex 05, France Centre for Hydrology and Ecology, Maclean Building, Oxon OX10 8BB, UK.
  • Grubhoffer L; Faculty of Science, University of South Bohemia and Biology Centre, Institute of Parasitology, Czech Academy of Sciences, 37005 Ceské Budejovice (Budweis), Czech Republic.
  • Fazakerley JK; The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK.
  • Kohl A; MRC - University of Glasgow Centre for Virus Research, Glasgow G11 5JR, UK The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK alain.kohl@glasgow.ac.uk.
Nucleic Acids Res ; 42(14): 9436-46, 2014 Aug.
Article en En | MEDLINE | ID: mdl-25053841
Arboviruses are transmitted by distantly related arthropod vectors such as mosquitoes (class Insecta) and ticks (class Arachnida). RNA interference (RNAi) is the major antiviral mechanism in arthropods against arboviruses. Unlike in mosquitoes, tick antiviral RNAi is not understood, although this information is important to compare arbovirus/host interactions in different classes of arbovirus vectos. Using an Ixodes scapularis-derived cell line, key Argonaute proteins involved in RNAi and the response against tick-borne Langat virus (Flaviviridae) replication were identified and phylogenetic relationships characterized. Analysis of small RNAs in infected cells showed the production of virus-derived small interfering RNAs (viRNAs), which are key molecules of the antiviral RNAi response. Importantly, viRNAs were longer (22 nucleotides) than those from other arbovirus vectors and mapped at highest frequency to the termini of the viral genome, as opposed to mosquito-borne flaviviruses. Moreover, tick-borne flaviviruses expressed subgenomic flavivirus RNAs that interfere with tick RNAi. Our results characterize the antiviral RNAi response in tick cells including phylogenetic analysis of genes encoding antiviral proteins, and viral interference with this pathway. This shows important differences in antiviral RNAi between the two major classes of arbovirus vectors, and our data broadens our understanding of arthropod antiviral RNAi.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ixodes / Interferencia de ARN / Virus de la Encefalitis Transmitidos por Garrapatas Límite: Animals Idioma: En Revista: Nucleic Acids Res Año: 2014 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ixodes / Interferencia de ARN / Virus de la Encefalitis Transmitidos por Garrapatas Límite: Animals Idioma: En Revista: Nucleic Acids Res Año: 2014 Tipo del documento: Article Pais de publicación: Reino Unido