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Characterization of type II thioesterases involved in natamycin biosynthesis in Streptomyces chattanoogensis L10.
Wang, Yue-Yue; Ran, Xin-Xin; Chen, Wei-Bin; Liu, Shui-Ping; Zhang, Xiao-Sheng; Guo, Yuan-Yang; Jiang, Xin-Hang; Jiang, Hui; Li, Yong-Quan.
Afiliación
  • Wang YY; College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
  • Ran XX; College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
  • Chen WB; College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
  • Liu SP; College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
  • Zhang XS; College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
  • Guo YY; College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
  • Jiang XH; College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
  • Jiang H; College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China,; Key Laboratory of Microbial Biochemistry and Metabolism Engineering of Zhejiang Province, Hangzhou, Zhejiang 310058, China. Electronic address: jianghuisioc@zjuedu.cn.
  • Li YQ; College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China,; Key Laboratory of Microbial Biochemistry and Metabolism Engineering of Zhejiang Province, Hangzhou, Zhejiang 310058, China. Electronic address: lyq@zju.edu.cn.
FEBS Lett ; 588(17): 3259-64, 2014 Aug 25.
Article en En | MEDLINE | ID: mdl-25064840
The known functions of type II thioesterases (TEIIs) in type I polyketide synthases (PKSs) include selecting of starter acyl units, removal of aberrant extender acyl units, releasing of final products, and dehydration of polyketide intermediates. In this study, we characterized two TEIIs (ScnI and PKSIaTEII) from Streptomyces chattanoogensis L10. Deletion of scnI in S. chattanoogensis L10 decreased the natamycin production by about 43%. Both ScnI and PKSIaTEII could remove acyl units from the acyl carrier proteins (ACPs) involved in the natamycin biosynthesis. Our results show that the TEII could play important roles in both the initiation step and the elongation steps of a polyketide biosynthesis; the intracellular TEIIs involved in different biosynthetic pathways could complement each other.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptomyces / Tioléster Hidrolasas / Natamicina / Ácido Graso Sintasas Idioma: En Revista: FEBS Lett Año: 2014 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptomyces / Tioléster Hidrolasas / Natamicina / Ácido Graso Sintasas Idioma: En Revista: FEBS Lett Año: 2014 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido