Your browser doesn't support javascript.
loading
Development of a multiplex Luminex assay for detecting swine antibodies to structural and nonstructural proteins of foot-and-mouth disease virus in Taiwan.
Chen, Tsu-Han; Lee, Fan; Lin, Yeou-Liang; Pan, Chu-Hsiang; Shih, Chia-Ni; Tseng, Chun-Hsien; Tsai, Hsiang-Jung.
Afiliación
  • Chen TH; Animal Health Research Institute, New Taipei City, Taiwan; Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
  • Lee F; Animal Health Research Institute, New Taipei City, Taiwan.
  • Lin YL; Animal Health Research Institute, New Taipei City, Taiwan.
  • Pan CH; Animal Health Research Institute, New Taipei City, Taiwan.
  • Shih CN; Animal Health Research Institute, New Taipei City, Taiwan.
  • Tseng CH; Animal Health Research Institute, New Taipei City, Taiwan.
  • Tsai HJ; Animal Health Research Institute, New Taipei City, Taiwan; Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan. Electronic address: tsaihj@ntu.edu.tw.
J Microbiol Immunol Infect ; 49(2): 196-207, 2016 Apr.
Article en En | MEDLINE | ID: mdl-25074628
ABSTRACT
BACKGROUND/PURPOSE(S) Foot-and-mouth disease (FMD) and swine vesicular disease (SVD) are serious vesicular diseases that have devastated swine populations throughout the world. The aim of this study was to develop a multianalyte profiling (xMAP) Luminex assay for the differential detection of antibodies to the FMD virus of structural proteins (SP) and nonstructural proteins (NSP).

METHODS:

After the xMAP was optimized, it detected antibodies to SP-VP1 and NSP-3ABC of the FMD virus in a single serum sample. These tests were also compared with 3ABC polypeptide blocking enzyme-linked immunosorbent assay (ELISA) and virus neutralization test (VNT) methods for the differential diagnosis and assessment of immune status, respectively.

RESULTS:

To detect SP antibodies in 661 sera from infected naïve pigs and vaccinated pigs, the diagnostic sensitivity (DSn) and diagnostic specificity (DSp) of the xMAP were 90.0-98.7% and 93.0-96.5%, respectively. To detect NSP antibodies, the DSn was 90% and the DSp ranged from 93.3% to 99.1%. The xMAP can detect the immune response to SP and NSP as early as 4 days postinfection and 8 days postinfection, respectively. Furthermore, the SP and NSP antibodies in all 15 vaccinated but unprotected pigs were detected by xMAP. A comparison of SP and NSP antibodies detected in the sera of the infected samples indicated that the results from the xMAP had a high positive correlation with results from the VNT and a 3ABC polypeptide blocking ELISA assay. However, simultaneous quantitation detected that xMAP had no relationship with the VNT. Furthermore, the specificity was 93.3-94.9% with 3ABC polypeptide blocking ELISA for the FMDV-NSP antibody.

CONCLUSION:

The results indicated that xMAP has the potential to detect antibodies to FMDV-SP-VP1 and NSP-3ABC and to distinguish FMDV-infected pigs from pigs infected with the swine vesicular disease virus.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Porcinos / Inmunoensayo / Proteínas no Estructurales Virales / Virus de la Fiebre Aftosa / Proteínas de la Cápside / Fiebre Aftosa / Anticuerpos Antivirales Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: J Microbiol Immunol Infect Asunto de la revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Taiwán

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Porcinos / Inmunoensayo / Proteínas no Estructurales Virales / Virus de la Fiebre Aftosa / Proteínas de la Cápside / Fiebre Aftosa / Anticuerpos Antivirales Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: J Microbiol Immunol Infect Asunto de la revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Taiwán