Development trends for generation of single-chain antibody fragments.
Immunopharmacol Immunotoxicol
; 36(5): 297-308, 2014 Oct.
Article
en En
| MEDLINE
| ID: mdl-25081575
Recombinant antibodies are increasingly being employed as therapeutic agents especially in combination with anti-cancer drugs. The single-chain antibody fragments are small antigen-binding proteins which provide the most commonly used antibody formats for diagnostic and therapeutic purposes. These antibody fragments have more rapid tumor penetration and clearance from the serum relative to full-length monoclonal antibodies. There are in vitro antibody-display technologies such as phage display, cell surface display, ribosome display and mRNA display that can be used to isolate high specificity and affinity single-chain antibodies against a wide variety of targets. We review these strategies for generation of stable and active antibody fragments in the present article.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fragmentos de Inmunoglobulinas
/
Anticuerpos de Cadena Única
/
Factores Inmunológicos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Immunopharmacol Immunotoxicol
Asunto de la revista:
ALERGIA E IMUNOLOGIA
/
FARMACOLOGIA
/
TOXICOLOGIA
Año:
2014
Tipo del documento:
Article
Pais de publicación:
Reino Unido