A novel, broadly applicable approach to isolation of fungi in diverse growth media.
J Microbiol Methods
; 105: 155-61, 2014 Oct.
Article
en En
| MEDLINE
| ID: mdl-25093757
ABSTRACT
Creatinine (CRN) is a vertebrate metabolic waste product normally found in blood and urine. Previous work demonstrated that the hydrochloride salt of creatinine (CRN-HCl) acted as a potent inhibitor of bacterial replication. Creatinine hydrochloride does not inhibit the growth of yeasts or molds (i.e. fungi), making it a potentially useful addition to growth media to facilitate isolation of environmental or clinically relevant fungal species. Sabouraud dextrose agar is the current medium of choice for detection and isolation of fungi although it does not offer optimal nutritional requirements for some fungi and can permit growth of bacteria which may subsequently inhibit fungal growth and/or obscure fungal isolation. We show that CRN-HCl effectively suppresses bacterial growth in either liquid or solid agar media while allowing outgrowth of slower growing fungi using either experimentally prepared samples or environmental samples.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas Microbiológicas
/
Medios de Cultivo
/
Hongos
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
J Microbiol Methods
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos