Microseed matrix screening for optimization in protein crystallization: what have we learned?
Acta Crystallogr F Struct Biol Commun
; 70(Pt 9): 1117-26, 2014 Sep.
Article
en En
| MEDLINE
| ID: mdl-25195878
ABSTRACT
Protein crystals obtained in initial screens typically require optimization before they are of X-ray diffraction quality. Seeding is one such optimization method. In classical seeding experiments, the seed crystals are put into new, albeit similar, conditions. The past decade has seen the emergence of an alternative seeding strategy microseed matrix screening (MMS). In this strategy, the seed crystals are transferred into conditions unrelated to the seed source. Examples of MMS applications from in-house projects and the literature include the generation of multiple crystal forms and different space groups, better diffracting crystals and crystallization of previously uncrystallizable targets. MMS can be implemented robotically, making it a viable option for drug-discovery programs. In conclusion, MMS is a simple, time- and cost-efficient optimization method that is applicable to many recalcitrant crystallization problems.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Acta Crystallogr F Struct Biol Commun
Año:
2014
Tipo del documento:
Article
País de afiliación:
Suiza