Fast-atom bombardment mass spectrometry of conjugated benzo(a)pyrene metabolites.

ABSTRACT

Fast-atom bombardment mass spectrometry has been used to obtain spectra of conjugated benzo(a)pyrene (bap) metabolites using a 11, glycerol + thioglycerol matrix, bap Glucuronides give positive- and negative-ion spectra with peaks due to [M + H]+ and [M - H]- ions and a major fragment peak (base peak) at [bap-OH]+ and [bap-O]-. bap Sulfates (sodium salts) give similar negative-ion spectra with [M - Na]- and [bap-O]- peaks, but the positive-ion spectra are dominated by sodium and glycerol adducts of the bap sulfates.