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Influence of oxygen on wound healing dynamics in healing-impaired diabetic mice.
Sano, Hitomi; Ichioka, Shigeru.
Afiliación
  • Sano H; Department of Surgical Science, Graduate School of Medicine, University of Tokyo , Tokyo , Japan.
J Plast Surg Hand Surg ; 49(3): 135-40, 2015 Jun.
Article en En | MEDLINE | ID: mdl-25272311
ABSTRACT
A previous experiment using an in vivo mouse model has proved that hypoxia increased angiogenesis during wound healing. It was hypothesised that one of the mechanisms for wound healing impairment in diabetes includes insufficient angiogenic ability in response to hypoxia. The current study aims to investigate the influence of hypoxia on wound healing in diabetic mice. Oxygen-impermeable (hypoxic group) and -permeable membranes (normoxic group) were used to control topical oxygen tension. Membranes were applied to symmetrical excisional wounds on diabetic mice. Wound area, granulated tissue thickness, and vascular density were analyzed. As results, a decrease in wound size on day 7 was observed in the normoxic group (20.7 ± 3.64%) compared with the hypoxic group (34.1 ± 4.98%). The normoxic group also showed significantly thicker granulated tissue than the hypoxic group (225.7 ± 54.7 vs 128.7 ± 42.4 µm). There was no significant difference in mean vascular density between normoxic and hypoxic groups (0.046 ± 0.022 vs 0.038 ± 0.017 mm(2)/mm(2), p = 0.80). Contrary to healthy mice, diabetic mice have shown no enhancement of angiogenesis in hypoxic condition. The findings illustrate that neovascularisation in response to hypoxia is diminished in diabetic wounds.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxígeno / Úlcera Cutánea / Cicatrización de Heridas / Neovascularización Fisiológica / Diabetes Mellitus Tipo 2 / Hipoxia Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Plast Surg Hand Surg Año: 2015 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxígeno / Úlcera Cutánea / Cicatrización de Heridas / Neovascularización Fisiológica / Diabetes Mellitus Tipo 2 / Hipoxia Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Plast Surg Hand Surg Año: 2015 Tipo del documento: Article País de afiliación: Japón