Coassembly and coupling of SK2 channels and mGlu5 receptors.
J Neurosci
; 34(44): 14793-802, 2014 Oct 29.
Article
en En
| MEDLINE
| ID: mdl-25355231
ABSTRACT
Group I metabotropic glutamate (mGlu) receptors regulate hippocampal CA1 pyramidal neuron excitability via Ca(2+) wave-dependent activation of small-conductance Ca(2+)-activated K(+) (SK) channels. Here, we show that mGlu5 receptors and SK2 channels coassemble in heterologous coexpression systems and in rat brain. Further, in cotransfected cells or rat primary hippocampal neurons, mGlu5 receptor stimulation activated apamin-sensitive SK2-mediated K(+) currents. In addition, coexpression of mGlu5 receptors and SK2 channels promoted plasma membrane targeting of both proteins and correlated with increased mGlu5 receptor function that was unexpectedly blocked by apamin. These results demonstrate a reciprocal functional interaction between mGlu5 receptors and SK2 channels that reflects their molecular coassembly.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Canales de Potasio de Pequeña Conductancia Activados por el Calcio
/
Receptor del Glutamato Metabotropico 5
/
Hipocampo
/
Neuronas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Neurosci
Año:
2014
Tipo del documento:
Article