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Generation of pluripotent stem cells without the use of genetic material.
Higuchi, Akon; Ling, Qing-Dong; Kumar, S Suresh; Munusamy, Murugan A; Alarfaj, Abdullah A; Chang, Yung; Kao, Shih-Hsuan; Lin, Ke-Chen; Wang, Han-Chow; Umezawa, Akihiro.
Afiliación
  • Higuchi A; 1] Department of Chemical and Materials Engineering, National Central University, Jhongli, Taiwan [2] Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia.
  • Ling QD; 1] Institute of Systems Biology and Bioinformatics, National Central University, Jhongli, Taiwan [2] Cathay Medical Research Institute, Cathay General Hospital, Taipei, Taiwan.
  • Kumar SS; Department of Medical Microbiology and Parasitology, Universities Putra Malaysia, Slangor, Malaysia.
  • Munusamy MA; Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia.
  • Alarfaj AA; Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia.
  • Chang Y; Department of Chemical Engineering, R&D Center for Membrane Technology, Chung Yuan Christian University, Jhongli, Taiwan.
  • Kao SH; Department of Chemical and Materials Engineering, National Central University, Jhongli, Taiwan.
  • Lin KC; Department of Chemical and Materials Engineering, National Central University, Jhongli, Taiwan.
  • Wang HC; Department of Obstetrics and Gynecology, Hungchi Women and Children's Hospital, Jhongli, Taiwan.
  • Umezawa A; Department of Reproduction, National Research Institute for Child Health and Development, Tokyo, Japan.
Lab Invest ; 95(1): 26-42, 2015 Jan.
Article en En | MEDLINE | ID: mdl-25365202
ABSTRACT
Induced pluripotent stem cells (iPSCs) provide a platform to obtain patient-specific cells for use as a cell source in regenerative medicine. Although iPSCs do not have the ethical concerns of embryonic stem cells, iPSCs have not been widely used in clinical applications, as they are generated by gene transduction. Recently, iPSCs have been generated without the use of genetic material. For example, protein-induced PSCs and chemically induced PSCs have been generated by the use of small and large (protein) molecules. Several epigenetic characteristics are important for cell differentiation; therefore, several small-molecule inhibitors of epigenetic-modifying enzymes, such as DNA methyltransferases, histone deacetylases, histone methyltransferases, and histone demethylases, are potential candidates for the reprogramming of somatic cells into iPSCs. In this review, we discuss what types of small chemical or large (protein) molecules could be used to replace the viral transduction of genes and/or genetic reprogramming to obtain human iPSCs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Pluripotentes Aspecto: Ethics Límite: Animals / Humans Idioma: En Revista: Lab Invest Año: 2015 Tipo del documento: Article País de afiliación: Arabia Saudita
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Pluripotentes Aspecto: Ethics Límite: Animals / Humans Idioma: En Revista: Lab Invest Año: 2015 Tipo del documento: Article País de afiliación: Arabia Saudita