Osthole induces apoptosis, suppresses cell-cycle progression and proliferation of cancer cells.
Anticancer Res
; 34(11): 6473-80, 2014 Nov.
Article
en En
| MEDLINE
| ID: mdl-25368248
ABSTRACT
BACKGROUND:
The aim of the present study was to determine the effects of osthole on cell proliferation and viability, cell-cycle progression and induction of apoptosis in human laryngeal cancer RK33 and human medulloblastoma TE671 cell lines. MATERIALS ANDMETHODS:
Cell viability was measured by means of the MTT method and cell proliferation by the 5-bromo-2-deoxyuridine (BrdU) incorporation assay. Cell-cycle progression was determined by flow cytometry, and induction of apoptosis by release of oligonucleosomes to the cytosol. The gene expression was estimated by a quantitative polymerase chain reaction (qPCR) method. High-performance counter-current chromatography (HPCCC) was applied for isolation of osthole from fruits of Mutellina purpurea.RESULTS:
Osthole decreased proliferation and cell viability of cancer cells in a dose-dependent manner. The tested compound induced apoptosis, increased the cell numbers in G1 and decreased cell number in S/G2 phases of the cell cycle, differentially regulating CDKN1A and TP53 gene expression depending on cancer cell type.CONCLUSION:
Osthole could be considered as a potential compound for cancer therapy and chemoprevention.Palabras clave
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ciclo Celular
/
Neoplasias Cerebelosas
/
Neoplasias Laríngeas
/
Apoptosis
/
Cumarinas
/
Proliferación Celular
/
Meduloblastoma
/
Antineoplásicos Fitogénicos
Límite:
Humans
Idioma:
En
Revista:
Anticancer Res
Año:
2014
Tipo del documento:
Article
País de afiliación:
Polonia