Stem cell injection in the hindlimb skeletal muscle enhances neurorepair in mice with spinal cord injury.
Regen Med
; 9(5): 579-91, 2014.
Article
en En
| MEDLINE
| ID: mdl-25372077
ABSTRACT
AIMS:
To develop a low-risk, little-invasive stem cell-based method to treat acute spinal cord injuries.methods:
Adult mice were submitted to an incomplete spinal cord injury, and mesenchymal stem cells injected intramuscularly into both hindlimbs. Behavior tests and MRI of the spinal cord were periodically performed for up to 6 months, along with immunohistochemical analysis. Immunohistochemical and PCR analysis of the muscles were used to detect the grafted cells as well as the soluble factors released.RESULTS:
The stem cell-treated mice presented significant improvements in their motor skills 5 months after treatment. Spinal cord repair was detected by magnetic resonance and immunohistochemistry. In the hindlimb muscles, the stem cells activated muscle and motor neuron repair mechanisms, due to the secretion of several neurotrophic factors.CONCLUSION:
Bone marrow mesenchymal stem cell injection into hindlimb muscles stimulates spinal cord repair in acute spinal cord lesions.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Traumatismos de la Médula Espinal
/
Trasplante de Médula Ósea
/
Trasplante de Células Madre Mesenquimatosas
/
Regeneración de la Medula Espinal
Límite:
Animals
Idioma:
En
Revista:
Regen Med
Año:
2014
Tipo del documento:
Article
País de afiliación:
España