Curcumin-induced histone acetylation inhibition improves stress-induced gastric ulcer disease in rats.
Mol Med Rep
; 11(3): 1911-6, 2015 Mar.
Article
en En
| MEDLINE
| ID: mdl-25405899
ABSTRACT
Curcumin is known to possess antiinflammatory properties. Despite the fact that curcumin is known to be a strong inhibitor of H+, K+ATPase activity, the mechanism underlying the curcumininduced inhibition of the transcription of the H+, K+ATPase α subunit in gastric mucosal parietal cells remains unclear. The present study investigated the possible mechanism by which curcumin inhibits stomach H+, K+ATPase activity during the acute phase of gastric ulcer disease. A rat model of stressinduced gastric ulcers was produced, in which the antiulcer effects of curcumin were examined. Curcumininduced inhibition of the H+, K+ATPase promoter via histone acetylation, was verified using a chromatin immunoprecipitation assay. The results showed that curcumin improved stressinduced gastric ulcer disease in rats, as demonstrated by increased pH values and reduced gastric mucosal hemorrhage and ulcer index. These effects were accompanied by a significant reduction in the level of histone H3 acetylation at the site of the H+, K+ATPase promoter and in the expression of the gastric H+,K+ATPase α subunit gene and protein. In conclusion, curcumin downregulated the acetylation of histone H3 at the site of the H+, K+ATPase promoter gene, thereby inhibiting the transcription and expression of the H+, K+ATPase gene. Curcumin was shown to have a preventive and therapeutic effect in gastric ulcer disease.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Úlcera Gástrica
/
Estrés Fisiológico
/
Estrés Psicológico
/
Histonas
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Curcumina
/
Antiinflamatorios
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Año:
2015
Tipo del documento:
Article