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Time point-based integrative analyses of deep-transcriptome identify four signal pathways in blastemal regeneration of zebrafish lower jaw.
Zhang, Hui; Wang, Xuelong; Lyu, Kailun; Gao, Siqi; Wang, Guan; Fan, Chunxin; Zhang, Xin A; Yan, Jizhou.
Afiliación
  • Zhang H; Department of Biology, Institute for Marine Biosystem and Neurosciences, College of Fisheries and Life Sciences, Shanghai Ocean University Shanghai, People's Republic of China.
Stem Cells ; 33(3): 806-18, 2015 Mar.
Article en En | MEDLINE | ID: mdl-25420467
ABSTRACT
There has been growing interest in applying tissue engineering to stem cell-based regeneration therapies. We have previously reported that zebrafish can faithfully regenerate complicated tissue structures through blastemal cell type conversions and tissue reorganization. To unveil the regenerative factors and engineering arts of blastemal regeneration, we conducted transcriptomal analyses at four time points corresponding to preamputation, re-epitheliation, blastemal formation, and respecification. By combining the hierarchical gene ontology term network, the DAVID annotation system, and Euclidean distance clustering, we identified four signaling pathways foxi1-foxo1b-pou3f1, pax3a-mant3a-col11/col2, pou5f1-cdx4-kdrl, and isl1-wnt11 PCP-sox9a. Results from immunohistochemical staining and promoter-driven transgenic fish suggest that these pathways, respectively, define wound epidermis reconstitution, cell type conversions, blastemal angiogenesis/vasculogenesis, and cartilage matrix-orientation. Foxi1 morpholino-knockdown caused expansions of Foxo1b- and Pax3a-expression in the basal layer-blastemal junction region. Moreover, foxi1 morphants displayed increased sox9a and hoxa2b transcripts in the embryonic pharyngeal arches. Thus, a Foxi1 signal switch is required to establish correct tissue patterns, including re-epitheliation and blastema formation. This study provides novel insight into a blastema regeneration strategy devised by epithelial cell transdifferentiation, blood vessel engineering, and cartilage matrix deposition.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración Ósea / Maxilares Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Stem Cells Año: 2015 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración Ósea / Maxilares Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Stem Cells Año: 2015 Tipo del documento: Article
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