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Direct study of fluorescently-labelled barley ß-glucan fate in an in vitro human colon digestion model.
Beeren, Sophie R; Christensen, Caspar E; Tanaka, Hidenori; Jensen, Morten G; Donaldson, Iain; Hindsgaul, Ole.
Afiliación
  • Beeren SR; Carlsberg Laboratory, Gamle Carlsberg Vej 10, 1799 Copenhagen V, Denmark. Electronic address: sophie.beeren@carlsberglab.dk.
  • Christensen CE; Carlsberg Laboratory, Gamle Carlsberg Vej 10, 1799 Copenhagen V, Denmark.
  • Tanaka H; Carlsberg Laboratory, Gamle Carlsberg Vej 10, 1799 Copenhagen V, Denmark.
  • Jensen MG; Carlsberg Research Center, Gamle Carlsberg Vej 4-6, 1799 Copenhagen V, Denmark.
  • Donaldson I; Carlsberg Research Center, Gamle Carlsberg Vej 4-6, 1799 Copenhagen V, Denmark.
  • Hindsgaul O; Carlsberg Laboratory, Gamle Carlsberg Vej 10, 1799 Copenhagen V, Denmark.
Carbohydr Polym ; 115: 88-92, 2015 Jan 22.
Article en En | MEDLINE | ID: mdl-25439872
ABSTRACT
ß-Glucans from cereals are ß(1-3)(1-4)-mixed linkage linear homopolysaccharides of D-glucopyranosyl residues, recently recognised as functional components of foods with benefits in maintaining the health of the digestive tract not least through a prebiotic effect. Here we describe the development of methodology to facilitate the study of ß-glucans as prebiotics. Relatively short ß-glucan fragments (DP 6-50) were produced by partial hydrolysis of ß-glucan fibres with Lichenase then functionalised at their reducing end with a tetramethylrhodamine dye. Their enzymatic break down by human colon microbiota in an in vitro fermentation model was examined. Digestion products were isolated by virtue of their fluorescence labels, identified and characterised using capillary electrophoresis and mass spectrometry. Complete digestion of the labelled substrates was indicated, as fluorescently labelled glucose was obtained as the final product. Furthermore, a pathway of enzymatic breakdown was proposed on the basis of a time course experiment; initial fast hydrolysis with an endo-1,3(4)-ß-glucanase was followed by slow degradation with an exo-1,4-ß-glucanase and finally slow action of an exo-1,3-ß-glucanase.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hordeum / Colon / Beta-Glucanos / Digestión / Fluorescencia / Modelos Biológicos Límite: Humans Idioma: En Revista: Carbohydr Polym Año: 2015 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hordeum / Colon / Beta-Glucanos / Digestión / Fluorescencia / Modelos Biológicos Límite: Humans Idioma: En Revista: Carbohydr Polym Año: 2015 Tipo del documento: Article