A novel packing arrangement of AcrB in the lipid bilayer membrane.
FEBS Lett
; 588(24): 4776-83, 2014 Dec 20.
Article
en En
| MEDLINE
| ID: mdl-25451234
ABSTRACT
The central component AcrB of the Escherichia coli drug efflux complex AcrA-AcrB-TolC has been extensively investigated by X-ray crystallography of detergent-protein 3-D crystals. In these crystals, AcrB packs as trimers - the functional unit. We visualized the AcrB-AcrB interaction in its native environment by examining E. coli lipid reconstituted 2-D crystals, which were overwhelmingly formed by asymmetric trimers stabilized by strongly-interacting monomers from adjacent trimers. Most interestingly, we observed lattices formed by an arrangement of AcrB monomers distinct from that in traditional trimers. This hitherto unobserved packing, might play a role in the biogenesis of trimeric AcrB.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Membrana Celular
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Proteínas de Escherichia coli
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Proteínas Asociadas a Resistencia a Múltiples Medicamentos
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Multimerización de Proteína
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Membrana Dobles de Lípidos
Idioma:
En
Revista:
FEBS Lett
Año:
2014
Tipo del documento:
Article
País de afiliación:
Nueva Zelanda
Pais de publicación:
ENGLAND
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ESCOCIA
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GB
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GREAT BRITAIN
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INGLATERRA
/
REINO UNIDO
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SCOTLAND
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UK
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UNITED KINGDOM