Testis mediated gene transfer: in vitro transfection in goat testis by electroporation.
Gene
; 554(1): 96-100, 2015 Jan 01.
Article
en En
| MEDLINE
| ID: mdl-25455104
ABSTRACT
Testis mediated gene transfer (TMGT) is a potential tool for making transgenic mice having more than 90% success rate. However, this method needs further standardization before it can be adapted in other species including livestock. In order to standardize the TMGT in goat, buck testes (n=20) collected from the slaughter house were injected with a vector driving green fluorescent protein (GFP) expression under a cytomegalovirus (CMV) promoter. Then, the testes were subjected to electroporation with predetermined voltage, pulse length, pulse interval and number of pulses. Seminiferous tubules were isolated from the electroporated testis and cultured in-vitro. The expression was checked at regular intervals. Green fluorescence was observed on different days in different samples. It suggests transient integration of the plasmid into the seminiferous tubules. This in-vitro transfection of seminiferous tubule using electroporation will provide valuable baseline information.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Túbulos Seminíferos
/
Testículo
/
Técnicas de Transferencia de Gen
/
Electroporación
Límite:
Animals
Idioma:
En
Revista:
Gene
Año:
2015
Tipo del documento:
Article