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Telomere elongation and naive pluripotent stem cells achieved from telomerase haplo-insufficient cells by somatic cell nuclear transfer.
Sung, Li-Ying; Chang, Wei-Fang; Zhang, Qian; Liu, Chia-Chia; Liou, Jun-Yang; Chang, Chia-Chun; Ou-Yang, Huan; Guo, Renpeng; Fu, Haifeng; Cheng, Winston T K; Ding, Shih-Torng; Chen, Chuan-Mu; Okuka, Maja; Keefe, David L; Chen, Y Eugene; Liu, Lin; Xu, Jie.
Afiliación
  • Sung LY; Institute of Biotechnology, National Taiwan University, Taipei, 106 Taiwan, ROC; Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115 Taiwan, ROC; Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei, 100 Taiwan, ROC.
  • Chang WF; Institute of Biotechnology, National Taiwan University, Taipei, 106 Taiwan, ROC.
  • Zhang Q; State Key Laboratory of Medicinal Chemical Biology and Collaborative Innovation Center for Biotherapy, Department of Cell Biology and Genetics, College of Life Sciences, Nankai University, Tianjin 300071, China.
  • Liu CC; Institute of Biotechnology, National Taiwan University, Taipei, 106 Taiwan, ROC; Institute of Cellular and System Medicine, National Health Research Institutes, Zhunan, 350 Taiwan, ROC.
  • Liou JY; Institute of Cellular and System Medicine, National Health Research Institutes, Zhunan, 350 Taiwan, ROC.
  • Chang CC; Institute of Biotechnology, National Taiwan University, Taipei, 106 Taiwan, ROC.
  • Ou-Yang H; Institute of Biotechnology, National Taiwan University, Taipei, 106 Taiwan, ROC.
  • Guo R; State Key Laboratory of Medicinal Chemical Biology and Collaborative Innovation Center for Biotherapy, Department of Cell Biology and Genetics, College of Life Sciences, Nankai University, Tianjin 300071, China.
  • Fu H; State Key Laboratory of Medicinal Chemical Biology and Collaborative Innovation Center for Biotherapy, Department of Cell Biology and Genetics, College of Life Sciences, Nankai University, Tianjin 300071, China.
  • Cheng WTK; Institute of Biotechnology, National Taiwan University, Taipei, 106 Taiwan, ROC; Department of Animal Science and Biotechnology, Tunghai University, Taichung, 407 Taiwan, ROC.
  • Ding ST; Institute of Biotechnology, National Taiwan University, Taipei, 106 Taiwan, ROC.
  • Chen CM; Department of Life Sciences, and Agricultural Biotechnology Center, National Chung Hsing University, Taichung, 402 Taiwan, ROC; Rong-Hsing Translational Medicine Center and iEGG Center, National Chung Hsing University, Taichung, 402 Taiwan, ROC.
  • Okuka M; Department of Obstetrics and Gynecology, University of South Florida College of Medicine, Tampa, FL 33612, USA.
  • Keefe DL; Department of Obstetrics and Gynecology, New York University Langone Medical Center, New York, NY 10016, USA.
  • Chen YE; Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center, Ann Arbor, MI 48109, USA.
  • Liu L; State Key Laboratory of Medicinal Chemical Biology and Collaborative Innovation Center for Biotherapy, Department of Cell Biology and Genetics, College of Life Sciences, Nankai University, Tianjin 300071, China. Electronic address: liulin@nankai.edu.cn.
  • Xu J; Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center, Ann Arbor, MI 48109, USA. Electronic address: jiex@umich.edu.
Cell Rep ; 9(5): 1603-1609, 2014 Dec 11.
Article en En | MEDLINE | ID: mdl-25464850
ABSTRACT
Haplo-insufficiency of telomerase genes in humans leads to telomere syndromes such as dyskeratosis congenital and idiopathic pulmonary fibrosis. Generation of pluripotent stem cells from telomerase haplo-insufficient donor cells would provide unique opportunities toward the realization of patient-specific stem cell therapies. Recently, pluripotent human embryonic stem cells (ntESCs) have been efficiently achieved by somatic cell nuclear transfer (SCNT). We tested the hypothesis that SCNT could effectively elongate shortening telomeres of telomerase haplo-insufficient cells in the ntESCs with relevant mouse models. Indeed, telomeres of telomerase haplo-insufficient (Terc(+/-)) mouse cells are elongated in ntESCs. Moreover, ntESCs derived from Terc(+/-) cells exhibit naive pluripotency as evidenced by generation of Terc(+/-) ntESC clone pups by tetraploid embryo complementation, the most stringent test of naive pluripotency. These data suggest that SCNT could offer a powerful tool to reprogram telomeres and to discover the factors for robust restoration of telomeres and pluripotency of telomerase haplo-insufficient somatic cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Telómero / Células Madre Pluripotentes Inducidas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Cell Rep Año: 2014 Tipo del documento: Article
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Telómero / Células Madre Pluripotentes Inducidas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Cell Rep Año: 2014 Tipo del documento: Article