Protective effect against focal cerebral ischemia injury in acute phase of a novel invasive device for regional hypothermia.
J Chin Med Assoc
; 78(1): 67-75, 2015 Jan.
Article
en En
| MEDLINE
| ID: mdl-25467794
ABSTRACT
BACKGROUND:
Systemic hypothermia is considered beneficial to stroke patients. However, many complications ensue. The aim of this study was to evaluate the effect of a new invasive regional cooling device in cerebral ischemic injury in a rat model.METHODS:
After a pilot study confirming the efficiency of the cooling device, 15 adult male Sprague-Dawley rats, weighing 300-400 g, were randomly assigned into three groups cooling device applied at 14°C and at 26°C, and a sham group. Focal cerebral ischemic injury was achieved by electrocauterization of the left middle cerebral artery through craniectomy and temporal occlusion of both common carotid arteries for 3 hours. Within 30 minutes after the end of ischemic injury, the cooling device was inserted into the rat brain through a stereotactic frame to provide regional hypothermia for 2 hours. The rats were sacrificed immediately after the 2-hour regional hypothermia.RESULTS:
Although triphenyltetrazolium chloride staining showed smaller ischemic lesions in both the 26°C and 14°C groups compared to the control group, Fluoro Jade C staining showed no neuroprotective effects in the rostrum cerebral cortex in both groups. However, both triphenyltetrazolium chloride and Fluoro Jade C staining indicated significant beneficial effects in the caudal cerebral cortex in rats with cooling device applied at 26°C compared to the 14°C and control groups.CONCLUSION:
Our findings indicated that the device can effectively achieve regional hypothermia and could be beneficial for patients with cerebral ischemia during the acute phase.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Isquemia Encefálica
/
Hipotermia Inducida
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Chin Med Assoc
Asunto de la revista:
MEDICINA
Año:
2015
Tipo del documento:
Article