Inhibitory effects of various anticoagulants on the infectivity of human cytomegalovirus.
J Virol Methods
; 24(3): 301-6, 1989 Jun.
Article
en En
| MEDLINE
| ID: mdl-2547823
ABSTRACT
We assessed the extent to which the anticoagulants heparin, sodium citrate, Alsever's solution and ethylene diamine tetra-acetic acid (EDTA) (at standard low concentration or at high concentration as attained when only a small blood volume was collected into anticoagulant) inhibited the infectivity of human cytomegalovirus (HCMV). All four anticoagulants had minimal effects on cell-associated virus, but heparin at either low or high concentration and EDTA at high concentration reduced the titre of cell-free virus by 10-100 fold. The large inhibitory effects of heparin and EDTA on cell-free virus indicated that sodium citrate or Alsever's solution were preferable as anticoagulants when blood was collected for assay of CMV viraemia by virus isolation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Citomegalovirus
/
Anticoagulantes
Límite:
Humans
Idioma:
En
Revista:
J Virol Methods
Año:
1989
Tipo del documento:
Article
País de afiliación:
Reino Unido