Toxicity of extracts from disposable chopsticks, toothpicks, and paper cups on L-929 cells.
Can J Physiol Pharmacol
; 93(4): 223-6, 2015 Apr.
Article
en En
| MEDLINE
| ID: mdl-25494822
ABSTRACT
OBJECTIVE:
To evaluate the toxicity of extracts from disposable chopsticks, toothpicks, and paper cups on L-929 cells.METHODS:
We followed national standards to prepare the extracts from disposable chopsticks, toothpicks, and paper cups used for the cell culture media, and the morphology of L-929 cells was observed with an optical microscope. The loss rate for adherent cells was evaluated with the trypan blue exclusion method, and cell proliferation was determined using the WST-1 assay.RESULTS:
Compared with the control group, the cells cultured in media containing the extracts showed signs of apoptosis and necrosis after culturing for 4 or 7 days, and the loss rate for adherent cells was significantly increased (P < 0.05). An obvious decrease in cell viability was also observed (P < 0.05).CONCLUSION:
The extracts from disposable chopsticks, toothpicks, and paper cups can affect the growth and proliferation of L-929 cells and are potentially toxic to humans.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Papel
/
Madera
/
Extractos Vegetales
/
Apoptosis
/
Seguridad de Productos para el Consumidor
/
Utensilios de Comida y Culinaria
/
Fibroblastos
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
Can J Physiol Pharmacol
Año:
2015
Tipo del documento:
Article