Molecular level evaluation on HEMA interaction with a collagen model.
Dent Mater
; 31(2): 88-92, 2015 Feb.
Article
en En
| MEDLINE
| ID: mdl-25499247
ABSTRACT
OBJECTIVE:
2-Hydroxyethylmethacrylate (HEMA) diffuses in wet dentin and promotes adhesion during dentin priming and bonding. We have investigated the molecular level interaction between HEMA and a collagen model by using saturation transfer difference (STD) NMR.METHODS:
The binding of HEMA to collagen was preliminarily investigated by suspending demineralized human dentin powders in a 4mM HEMA solution for 1h and measuring the decrease in the HEMA concentration on a spectrophotometer. The molecular level interaction of HEMA with atelocollagen, which was used as a collagen model, was investigated by STD-NMR spectroscopy.RESULTS:
The HEMA concentration in the suspension did not change, indicating that HEMA did not bind to dentin collagen. This was confirmed by STD-NMR; when the atelocollagen resonance was saturated, no saturation was propagated to HEMA and no STD signals were detected.SIGNIFICANCE:
The HEMA protons were not near the atelocollagen surface, indicating HEMA did not interact with atelocollagen. The collagen fibrils may be surrounded by water molecules in dentin/bond interfaces, which prevent the direct HEMA binding interaction.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectroscopía de Resonancia Magnética
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Colágeno
/
Recubrimientos Dentinarios
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Dentina
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Metacrilatos
Límite:
Humans
Idioma:
En
Revista:
Dent Mater
Asunto de la revista:
ODONTOLOGIA
Año:
2015
Tipo del documento:
Article