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The identification of oppA gene homologues as part of the oligopeptide transport system in mycoplasmas.
Wium, Martha; Botes, Annelise; Bellstedt, Dirk U.
Afiliación
  • Wium M; Department of Biochemistry, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa.
  • Botes A; Department of Biochemistry, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa. Electronic address: annelise@sun.ac.za.
  • Bellstedt DU; Department of Biochemistry, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa.
Gene ; 558(1): 31-40, 2015 Mar 01.
Article en En | MEDLINE | ID: mdl-25528211
ABSTRACT
The lack of an annotated oppA gene as part of many oligopeptide permease (opp) operons has questioned the necessity of the oligopeptide-binding domain (OppA) as a part of the Opp transport system in mycoplasmas. This study investigated the occurrence of an oppA gene as part of the oppBCDF operon in 42 mycoplasma genomes. Except for hemoplasma, all mycoplasmas were found to possess one or more copies of the oppBCDF operon and with the help of similarity searches their oppA genes could be identified. Phylogenetic analysis of the combined OppABCDF amino acid sequences allowed them to be grouped into three types. Each type has a unique set of conserved motifs, which are likely to reflect substrate preference and adaption strategies. Our approach allowed the identification of oppA gene homologues for all mycoplasma opp operons and thereby provides a method for re-evaluating the current annotation of oppA genes in mycoplasma genomes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Proteínas Portadoras / Lipoproteínas / Mycoplasma Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Gene Año: 2015 Tipo del documento: Article País de afiliación: Sudáfrica

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Proteínas Portadoras / Lipoproteínas / Mycoplasma Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Gene Año: 2015 Tipo del documento: Article País de afiliación: Sudáfrica