Evaluation and comparison of alpha- and beta-amanitin toxicity on MCF-7 cell line.
Turk J Med Sci
; 44(5): 728-32, 2014.
Article
en En
| MEDLINE
| ID: mdl-25539537
ABSTRACT
BACKGROUND/AIM:
Alpha- and beta-amanitins are the main toxins of the poisonous Amanita phalloides mushroom. Although there are many studies available concerning alpha-amanitin, there are limited data about beta-amanitin in the literature. Therefore, this study is aimed at comparing the toxic effects of alpha- and beta-amanitin on the MCF-7 cell line. MATERIALS ANDMETHODS:
The alpha- and beta-amanitins used for this research were purified from Amanita phalloides by preparative high-performance liquid chromatography. The MCF-7 breast cancer cell line was used, and specific concentrations of the toxins (100, 10, 1, 0.1, and 0.01 µg/mL) were applied to the cells. The MTT test was performed to determine the level of toxicity, and the quantity of protein in the cell was measured using the biuret test.RESULTS:
The aLpha-amanitin showed a higher toxicity at 36 h, while the highest inhibition of protein synthesis by the beta-amanitin was observed at 24 h.CONCLUSION:
It was shown that the beta-amanitin may be responsible for toxicity, like alpha-amanitin, in Amanita phalloides mushroom poisoning. The early inhibition of protein synthesis for beta-amanitin might be useful for future experiments and research.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Alfa-Amanitina
/
Amanitinas
Límite:
Humans
Idioma:
En
Revista:
Turk J Med Sci
Año:
2014
Tipo del documento:
Article