[Analytical studies on flavonoids constituents in Apocynum venetom leaves by UPLC-Q-TOF-MS].
Zhong Yao Cai
; 37(7): 1199-204, 2014 Jul.
Article
en Zh
| MEDLINE
| ID: mdl-25566657
ABSTRACT
OBJECTIVE:
To establish an ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometric (UPLC-Q-TOF-MS) method for analyzing the flavonoids constituents in Apocynum venetom leaves.METHODS:
The analysis was performed on an Alltima C18 analytical column with a gradient solvent system of acetonitrile-1.0% acetic acid at the flow rate of 0.8 mL/min. The detection wavelength was at 254 nm and the column temperature was at 30 degrees C. Identification and detection were collected under the negative electrospray ion mode using Q-TOF-MS.RESULTS:
11 flavonoids constituents being separated and identified, six of them were detected simultaneously. The results showed that the six detected flavonoids constituents had good linearity between concentrations and peak area (r > 0.9996), and the average recoveries of the method were between 94.3% - 103.3%.CONCLUSION:
The method is accurate, reproducible, and can be used for the quality control of Apocynum venetom leaves.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas
/
Flavonoides
/
Cromatografía Líquida de Alta Presión
/
Apocynum
Idioma:
Zh
Revista:
Zhong Yao Cai
Asunto de la revista:
TERAPIAS COMPLEMENTARES
Año:
2014
Tipo del documento:
Article
País de afiliación:
China