Optical imaging. Expansion microscopy.
Science
; 347(6221): 543-8, 2015 Jan 30.
Article
en En
| MEDLINE
| ID: mdl-25592419
ABSTRACT
In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. We discovered that by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call expansion microscopy (ExM). Thus, this process can be used to perform scalable superresolution microscopy with diffraction-limited microscopes. We demonstrate ExM with apparent ~70-nanometer lateral resolution in both cultured cells and brain tissue, performing three-color superresolution imaging of ~10(7) cubic micrometers of the mouse hippocampus with a conventional confocal microscope.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Invaginaciones Cubiertas de la Membrana Celular
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Imagen Óptica
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Hipocampo
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Microscopía
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Microtúbulos
Límite:
Animals
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Humans
Idioma:
En
Revista:
Science
Año:
2015
Tipo del documento:
Article
País de afiliación:
Estados Unidos