Functional characterization of MpaG', the O-methyltransferase involved in the biosynthesis of mycophenolic acid.
Chembiochem
; 16(4): 565-9, 2015 Mar 02.
Article
en En
| MEDLINE
| ID: mdl-25630520
ABSTRACT
Mycophenolic acid (MPA, 1) is a clinically important immunosuppressant. In this report, a gene cluster mpa' responsible for the biosynthesis of 1 was identified from Penicillium brevicompactum NRRL 864. The S-adenosyl-L-methionine-dependent (SAM-dependent) O-methyltransferase encoded by the mpaG' gene was functionally and kinetically characterized in vitro. MpaG' catalyzes the methylation of demethylmycophenolic acid (DMMPA, 6) to form 1. It also showed significant substrate flexibility by methylating two structural derivatives of 6 prepared by organic synthesis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Penicillium
/
Proteínas Fúngicas
/
Metiltransferasas
/
Ácido Micofenólico
Idioma:
En
Revista:
Chembiochem
Asunto de la revista:
BIOQUIMICA
Año:
2015
Tipo del documento:
Article