RNA and protein expression of HLA-A(∗)23:19Q.
Hum Immunol
; 76(4): 286-91, 2015 Apr.
Article
en En
| MEDLINE
| ID: mdl-25676117
ABSTRACT
The assignment of null alleles is clinically relevant in stem cell transplantation, in particular for donor selection. It is unclear how questionable (Q) alleles, having an unknown expression profile, should be considered in matching criteria. In this study we analyzed the RNA and protein expression profile of a questionable allele encountered in a sample of the Guadeloupe population GD23Q, HLA-A(∗)2319Q, 290201. Full-length DNA sequencing of HLA-A(∗)2319Q revealed a single polymorphism at position 619 (G>A) compared to HLA-A(∗)230101. Serological typing showed only the presence of HLA-A29; HLA-A(∗)2319Q was not detected on the cell surface. The absence of HLA-A(∗)2319Q surface expression was shown by flow cytometry using a directly labeled monoclonal antibody and a panel of five indirectly labeled polyclonal antibodies all directed against HLA-A23 (HLA-A9) molecules. Allele specific amplification revealed the absence of intact full-length mRNA, but the presence of two major alternatively spliced mRNAs sequencing identified that in one variant exon 3 is missing and in the other variant introns 2 and 3 are retained. Based upon the lack of HLA-A(∗)2319Q surface expression and the presence of aberrant mRNA transcripts only, this study shows that HLA-A(∗)2319Q is non-expressed.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Prueba de Histocompatibilidad
/
Antígenos HLA-A
/
Trasplante de Células Madre
/
Selección de Donante
Tipo de estudio:
Prognostic_studies
Límite:
Humans
País/Región como asunto:
Caribe
/
Guadalupe
Idioma:
En
Revista:
Hum Immunol
Año:
2015
Tipo del documento:
Article
País de afiliación:
Países Bajos