Isolation and identification of free amino acids as crystalline N-t-butyloxycarbonyl, O-phenacyl derivatives.

ABSTRACT

A general and efficient method for the quantitative isolation of free amino acids from natural sources and their identification as crystalline N-t-butyloxycarbonyl amino acid phenacyl esters is described. The applicability of this method is illustrated in the isolation and characterization of major free amino acids from the Mediterranean fruit fly Ceratitis capitata.