Analysis of the radiation-damage-free X-ray structure of photosystem II in light of EXAFS and QM/MM data.
Biochemistry
; 54(9): 1713-6, 2015 Mar 10.
Article
en En
| MEDLINE
| ID: mdl-25710258
ABSTRACT
A recent femtosecond X-ray diffraction study produced the first high-resolution structural model of the oxygen-evolving complex of photosystem II that is free of radiation-induced manganese reduction (Protein Data Bank entries 4UB6 and 4UB8 ). We find, however, that the model does not match extended X-ray absorption fine structure and QM/MM data for the S1 state. This is attributed to uncertainty about the positions of oxygen atoms that remain partially unresolved, even at 1.95 Å resolution, next to the heavy manganese centers. In addition, the photosystem II crystals may contain significant amounts of the S0 state, because of extensive dark adaptation prior to data collection.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Teoría Cuántica
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Complejo de Proteína del Fotosistema II
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Espectroscopía de Absorción de Rayos X
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Simulación de Dinámica Molecular
Idioma:
En
Revista:
Biochemistry
Año:
2015
Tipo del documento:
Article
País de afiliación:
Estados Unidos