EST-SSR markers from Heterodera glycines Ichinohe.
Genetika
; 50(10): 1259-62, 2014 Oct.
Article
en En
| MEDLINE
| ID: mdl-25720259
ABSTRACT
The soybean cyst nematode Heterodera glycines Ichinohe is a severe agricultural pest for which genetic resources are limited. In this study, 295 simple sequence repeats (SSRs) were identified from 259 expressed sequenced tags (ESTs), Which were selected from 9,443 unigenes. The successful primer pairs were designed against six regions. In total, 30 alleles were identified from 30 individuals using the six markers, with an average of five alleles per locus (range, 4-7). The observed and expected heterozygosities were 0.074-0.900 and 0.266-0.775, respectively. Significant departure from Hardy-Weinberg equilibrium was found at three of the six loci. The EST-based SSR markers developed in this study may contribute to better understanding of the genetic structure of H. glycines populations.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Repeticiones de Microsatélite
/
Etiquetas de Secuencia Expresada
/
Nematodos
Límite:
Animals
Idioma:
En
Revista:
Genetika
Año:
2014
Tipo del documento:
Article