Photoreceptor cells display a daily rhythm in the orphan receptor Esrrß.
Mol Vis
; 21: 173-84, 2015.
Article
en En
| MEDLINE
| ID: mdl-25737630
ABSTRACT
PURPOSE:
Nuclear orphan receptors are critical for the development and long-term survival of photoreceptor cells. In the present study, the expression of the nuclear orphan receptor Esrrß--a transcriptional regulator of energy metabolism that protects rod photoreceptors from dystrophy--was tested under daily regulation in the retina and photoreceptor cells.METHODS:
The daily transcript and protein amount profiles were recorded in preparations of the whole retina and microdissected photoreceptor cells using quantitative PCR (qPCR) and western blot analysis.RESULTS:
Esrrß displayed a daily rhythm with elevated values at night in the whole retina and enriched photoreceptor cells. Daily regulation of Esrrß mRNA depended on light input but not on melatonin, and evoked a corresponding rhythm in the Esrrß protein.CONCLUSIONS:
The data presented in this study indicate that daily regulation of Esrrß in photoreceptor cells may contribute to their adaptation to 24-h changes in metabolic demands.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Retina
/
ARN Mensajero
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Receptores de Estrógenos
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Ritmo Circadiano
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Células Fotorreceptoras Retinianas Bastones
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Metabolismo Energético
Límite:
Animals
Idioma:
En
Revista:
Mol Vis
Asunto de la revista:
BIOLOGIA MOLECULAR
/
OFTALMOLOGIA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
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US
/
USA